"We are very happy with the 13C, 15N-labeled
RNA nucleotides that we have obtained from Silantes. We consistently receive
samples of high purity that afford high yields when used in in vitro transcription
reactions in our lab. It is also significant that we have been able to
receive relatively large amounts of labeled ribo-NTPs with minimal minimal
delays from the time that orders are placed to the time the samples arrive
in our lab (typically less than 2-3 weeks)."
"The 13C, 15N labeled
NTPs provided by Silantes were of high quality and worked very well in
our hands. I can strongly recommend to use the products by Silantes."
"We are really pleased by the quality of the SILANTES 13C,
15N-labeled rNTPs. At a very resonable price, they reproducibely
give yields comparable to optimized in vitro transcription reactions using
commercially available unlabeled rNTPs. No further purification needed!
And equally important, SILANTES typically delivered with minimal delays
even when we ordered larger quantities."
"After purification, about 35 mg of pure protein were obtained with
Silantes OD2 DN Media. This is even a few milligrams more than we usually
get from LB cultures."
"Protein expression can be EASILY optimized in Silantes medium
and often the best conditions found in LB standard medium can be used.
Silantes saves your time! Silantes media are an excellent opportunity
to get labeled samples for MR studies especially if you need a fast preparation
for high through-put targets. Today most of the labeled samples of our
lab are prepared using Silantes Media."
"The result was comparable to typical rich growth media. In comparison
to glucose-minimal media, the yield on Silantes OD2 was two-times higher;
the growth parameters were good. Silantes OD2 media are very convenient
"We have used Silantes Expression media for protein expression.
The yields were with the first trial superior to our results using minimal
medium.I can strongly recommend to use the products by Silantes."
"We compared Silantes unlabeled media with Silantes CN labeled media
and found no substantial differences in growth or expression rates. We
have enclosed data of a typical growth curve and the resulting protein
yield obtained in the same system, using N-labeled M9 medium. Silantes
media give extraordinary high results compared to common M9 media."
Silantes GmbH, Gollierstrasse 70 c
Silantes GmbH I Gollierstr. 70 c I 80339 München I Germany