Yeast Extracts

Stable Isotope Labeled Yeast Extracts as Growth Media for SF9 Cells (Insect Cells)

Yeast extracts are dried and purified essences of yeast that are often used as a source of proteins, sugars, and essential growth factors. A yeast autolysate is an extract, in this case from the autolyzing yeast strain Sacharomyces cerevisiae, which is a good source of amino acids and vitamins, especially the water-soluble vitamin B complex. Compared to acid hydrolysis, gentle autolysis preserves the acid-labile amino acids.

Novel evidence shows that isotope-labeled yeast extract is an excellent source of amino acids for the insect cell/baculovirus expression system. By supplementing media with a well-considered amount of yeast extract, similar protein amounts as with full media are obtained, without compromising on isotope incorporation.[1]

Stable isotope labeled yeast autolysate from Silantes

The stable isotope labeled yeast extract from Silantes is ideally suited for use in culture media and for culturing various microorganisms. It provides uniformly isotope-labeled (> 98 atom %) multifunctional nutrient supplements that can also be used for microbial fermentation.

[1] Franke, B., Opitz, C., Isogai, S., Grahl, A., Delgado, L., Gossert, A. D., & Grzesiek, S. (2018). Production of isotope-labeled proteins in insect cells for NMR. Journal of Biomolecular NMR, 71(3), 173–184. https://doi.org/10.1007/s10858-018-0172-7