Phosphoramidites

 

BUY ISOTOPICALLY LABELED PHOSPHORAMIDITES IN OUR WEBSHOP

 

DEOXYRIBOPHOSPHORAMIDITES

These amidites are uniformly labeled (in the nucleotide part) and supplied as 13C- and 15N- labeled compounds. They are chemically synthesized from deoxynucleosides obtained from 13C- or 15N- labeled DNA or RNA, respectively. Click here to get further information about our deoxyribophosphoramidites at our webshop.

This organic-biochemical approach permits us to offer the deoxyphosphoramidites at very competitive prices.

 

RIBOPHOSPHORAMIDITES

These amidites are available site-specifically labeled in a variety of patterns of 2H, 13C and 15N labels. The available patterns are shown on the bottom of this page. Click here to get further information about our ribophosphoramidites at our webshop.

 

SUPPLY

The amidites are supplied in 10 mg and 50 mg quantities and delivered in vials suitable for chemical synthesis.

 

QUALITY

The isotopic enrichment of the amidites is >98 % with a chemical purity of >95 %. This is confirmed by mass spectrometric analysis and 31P-NMR (see below in figure 1). Figure 1 verifies the absence of phosphonate at 150ppm as a well-known contamination at the synthesis of deoxyribophosphoramidites.

 

Quality verification absence of phosphonate
Figure 1: 31P-NMR spectra as quality verification, absence of phosphonate at 150ppm

 

 

Silantes currently offers following productportfolio of phosphoramidites:

Deoxy-Phosphoramidites

       

Product #

Description

Isotope

Quantity

126303601

deoxyAdenosine Phosphoramidite (DMT-dA(Bz)-CE)

U-15N

10 mg

126303801

 

  50 mg

126303602

deoxyCytidine Phosphoramidite (DMT-dC(Ac)-CE)

U-15N

10 mg

126303802

   

50 mg

126303603

deoxyGuanosine Phosphoramidite (DMT-dG(dmf)-CE)

U-15N

10 mg

126303803

   

 50 mg

126303604

deoxyThymidine Phosphoramidite (DMT-dT-CE)

U-15N

10 mg

126303804

 

  50 mg

126603601

deoxyAdenosine Phosphoramidite (DMT-dA(Bz)-CE)

U-13C, 15N

10 mg

126603801

 

  50 mg

126603602

deoxyCytidine Phosphoramidite (DMT-dC(Ac)-CE)

U-13C, 15N

10 mg

126603802

 

  50 mg

126603603

deoxyGuanosine Phosphoramidite (DMT-dG(dmf)-CE)

U-13C, 15N

10 mg

126603803

 

  50 mg

126603604

deoxyThymidine Phosphoramidite (DMT-dT-CE)

U-13C, 15N

10 mg

126603804

 

  50 mg
       

Ribo-Phosphoramidites

       

Product #

Description

Isotope

Quantity

127213601

riboAdenosine Phosphoramidite (DMT-TBS-rA(Ac)-CE)

8-13C

10 mg

127213801

50 mg

   

127213603

riboGuanosine Phosphoramidite (DMT-TBS-rG(Ac)-CE)

8-13C

10 mg

127213803

 

  50 mg

127313601

riboAdenosine Phosphoramidite (DMT-TBS-rA(Ac)-CE)

1-15N

10 mg

127313801

 

  50 mg

127313602

riboCytidine Phosphoramidite (DMP-TBS-rC(Ac)-CE)

3-15N

10 mg

127313802

 

  50 mg

127313603

riboGuanosine Phosphoramidite (DMT-TBS-rG(Ac)-CE)

1-15N

10 mg

127313803

 

  50 mg

127313604

riboUridine Phosphoramidite (DMT-TBS-rU-CE)

3-15N

10 mg

127313804

 

  50 mg

127323602

riboCytidine Phosphoramidite (DMT-TBS-rC(Ac)-CE)

1-15N, 3-15N

10 mg

127323802

 

  50 mg

127323604

riboUridine Phosphoramidite (DMT-TBS-rU-CE)

1-15N, 3-15N

10 mg

127323804

 

  50 mg

127413602

riboCytidine Phosphoramidite (DMT-TBS-rC(Ac)-CE)

5-2H, 6-13C

10 mg

127413802

 

  50 mg

127413604

riboUridine Phosphoramidite (DMT-TBS-rU-CE)

5-2H, 6-13C

10 mg

127413804

 

  50 mg

 

 

Silantes also offers a custom synthesis service for deoxy- and ribophosphoramidites.

 

 

 To get more information or to order our phosphoramidites, please visit our webshop.