SILAC is based on metabolic labelling with stable isotopes in the cell culture medium and living animals. This enables accurate quantitative analysis of the cellular proteome and its posttranslational modifications (PTMs).
Although SILAC was initially developed as a cell culture- based technology, today a variety of model organisms such as worms, flies and mice can be labelled with stable isotopes. Another important aspect is the pulsed SILAC approach, in which cells and animals are labelled for specific periods of time in order to shed light on the dynamics of the proteome.
The lecture summarizes the principles of SILAC technology, its applications in biological research and the current status of this technology.
Watch the June 11, 2024 Webinar
Speaker: Prof. Dr. Martin Krüger
Head of Proteomics
CECAD Excellence in Aging Research
More information about Prof. Dr. Martin Krüger and his work
Research at Marcus Krüger Lab
The research focus of our group is to develop and apply quantitative proteomics methods in different biological areas. The aim of our study is the analysis of protein dynamics and post-translational modifications (PTMs) in cellular networks under regular and diseased conditions, such as type 2 diabetes mellitus and neurodegenerative disorders. We are also interested in transferring the SILAC technology to various model organisms, including worms, flies, zebrafish, and rodents.
Our group focusses on applying quantitative proteomics to analyse the dynamics of heart and skeletal muscle tissue. Skeletal muscles differ in their anatomical structure, neuronal innervation, and metabolic activity. Since muscle tissue make up approximately half of a person´s body weight its right function is essential for body shape, force production, and overall body homeostasis, including sugar uptake and energy production. In addition, skeletal muscle tissue is highly adaptable and is able to rapidly respond to environmental changes by modulating the synthesis and degradation of proteins.
Source: https://krueger.cecad-labs.uni-koeln.de/home
Curriculum Vitae Prof. Dr. Marcus Krüger
Academic education
- 1998–2002: Graduate studies in Chemistry, University of Halle-Wittenberg (Dissertation)
- 1991–1997: Studies in Chemistry and Biology, Technical University, Braunschweig
Scientific degree
- 2002: PhD in Chemistry, University of Halle-Wittenberg, Mentor: Prof. Dr. T. Braun
Professional experience
- Since 2014: Full Professor, Institute for Genetics/Cluster of Excellence on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne
- 2008–2014: Group Leader, Max-Planck-Institute for Heart and Lung Research, Bad Nauheim, Germany
Learn more about Prof. Dr. Krüger’s CV, publications and research projects.
