Custom RNA and DNA Oligonucleotide Synthesis Service

Oligonucleotides or oligos are short synthetic strands of DNA or RNA used in various applications in molecular biology and synthetic biology. Stable isotope labeling of oligonucleotides, a common method in quantitative mass spectrometry and NMR, involves incorporating stable isotopes such as ²H, ¹³C or ¹⁵N into synthetic or enzymatic oligonucleotides.

The stable isotopes act as molecular tags, allowing the behavior, location, and role of oligonucleotides in processes such as metabolism, gene expression, and replication to be tracked. Stable isotope labeling provides an alternative for detecting oligonucleotides in living systems. Labeled oligonucleotides can be detected using techniques such as mass spectrometry or nuclear magnetic resonance (NMR).

RNA and DNA Synthesis Services developed by Silantes

Silantes offers a currently unrivaled product line for isotope labeled RNA and DNA. With many years of experience, we are a reliable source for both, isotope-labeled RNA and DNA building blocks NTPs and phosphoramidites, as well as a range of services for oligonucleotide synthesis using isotope-labeled building blocks.

• Extensive knowledge of the special requirements for handling stable isotope-labeled reagents in oligonucleotide synthesis and
• Optimisation of oligonucleotide synthesis services with respect to minimal use of stable isotopes and small sample sizes.

This allows us to offer unmatched services and prices for stable isotope-labeled oligonucleotide synthesis and reagents. Dependent on isotope labeling specification and sequence length, Silantes has developed different RNA synthesis strategies for:

• Uniformly Labeled RNA Oligonucleotides
• Uniformly labeled DNA Oligonucleotides
• Site-Specifically labeled RNA & DNA Oligonucleotides
  

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Uniformly Labeled RNA Oligonucleotides

For synthesis of uniformly stable isotope-labeled (²H, ¹³C, ¹⁵N, ¹⁸O, ¹⁹F) RNA oligonucleotides, Silantes uses the T7 RNA polymerase system for in vitro transcription.

In order to make stable isotope-labeled RNA reagents affordable, Silantes has optimized the production process:

• Reasonably priced raw materials: the isotope-labeled rNTPs are made in-house by the company.
• Low purification costs: efficient synthesis using the T7 RNA polymerase system.

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Uniformly Labeled DNA Oligonucleotides

Silantes has developed a cost-effective technology for synthesizing stable isotope-labeled DNA oligonucleotides.
The enzymatic method differs from the conventional solid-phase synthesis and allows a more efficient conversion of isotope-labeled dNTPs into oligonucleotides, either isotope-labeling the entire oligonucleotide or all positions of a particular base.

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Site-Specifically labeled RNA & DNA Oligonucleotides

For site-specific incorporation of stable isotope-labeled (²H, ¹³C, ¹⁵N, ¹⁸O, ¹⁹F) nucleotides into RNA or DNA sequences solid-phase chemical synthesis is the method of choice.

For this purpose nucleoside phosphoramidites are used during the respective cycle of oligonucleotide synthesis.

Silantes offers both:

• Stable isotope-labeled ribo- and deoxy-phosphoramidites oligonucleotide synthesis building blocks
• Solid phase synthesis service for stable isotope-labeled oligonucleotides

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Advantages of Stable Isotope Labeling

The advantages of stable isotope labeling of nucleic acids are significant and contribute to various aspects of molecular biology research and applications. Some key advantages are:

• It is non-radioactive and safe for use in living organisms, allowing real-time studies of biological processes in their natural context
• It allows high-resolution detection by techniques such as mass spectrometry and NMR
• It can be applied to modified nucleotides, such as methylated or acetylated forms
• Fluorine labeling allows the study of RNA structure and interactions using ¹⁹F NMR

RNA therapeutics relevant reagents and services

Silantes offers stable isotope labeled products and services that are relevant to RNA therapeutics, including:

• mRNA synthesis
• Incorporation of methylated nucleic acids
• Incorporation of locked nucleic acids (LNAs)
• Incorporation of phosphorothioate linkers
• Quality control (qc) by mass spectrometry and hplc

Get your RNA & DNA synthesis project started with Silantes

To provide a quote for your RNA or DNA synthesis project, we need the following information:

• length and sequence of the oligonucleotide,
• synthesis method (uniform or site-specific labeling),
• modification,
• isotopic labeling (²H, ¹³C, ¹⁵N, ¹⁸O, ¹⁹F),
• desired quantity
• is purification desired? Depending on the sequence, Silantes experts will decide which method of purification (gel or HPLC) is best suited for your oligo nucleotide.

Based on this information, we will prepare a quotation for the synthesis of your RNA or DNA fragment. Once an order is placed, purified fragment delivery time is generally between 3 – 6 weeks. Request a quote: sales@silantes.com.