Cell Growth Media for Escherichia coli and Yeast Culture

• ¹³C- ECO Media powder for yeast
• ¹³C 15N – ECO Media powder for yeast

Rich Media compared to Minimal Media

A rich medium is a cell culture medium that contains, among other things, amino acids as well as glucose to promote protein expression.

A minimal medium, on the other hand, is a cell culture medium in which the only carbon source is glucose, glycerol or pyruvate, while the only nitrogen source is either ammonium chloride or ammonium sulphate.

In order to evaluate the cost-effectiveness of Silantes Rich media for your project, the respective costs of a corresponding minimal medium in relation to the protein yield can be used for comparison. We have validated that Silantes Rich Medium can increase protein expression by over 200% compared to Minimal Medium for numerous proteins.

Please contact us for a free sample to validate Silantes Rich Media for your application .

Boost the performance of Minimal Media with Silantes Rich Media

Stable isotope-labeled proteins are often expressed in minimal media (or “M9” media). As minimal media contains only essential nutrients required for growth, this can result in slower growth rates and lower biomass compared to rich media, ultimately leading to lower protein yields. This problem can be solved by supplementing the M9 medium with Silantes Rich Media.

Figure 1 demonstrates that 1 % w/v SILEX Powder Medium (or corresponding 1.7 % v/v OD10 Concentrate Medium) added as a supplement to M9 medium increases the initial cell growth rate and the final optical density.

High Standards in Quality Control

All our growth media have an isotope enrichment of >98 atom %. This specification is validated through mass spectrometry.

In addition, we ensure the biological integrity of our media by performing bacterial and yeast growth tests on each and every batch of media. Each delivery includes a quality certificate that includes all quality control results.

No Addition of D₂O or ¹³C-Glucose Needed

As the raw materials D₂O and ¹³C-Glucose become more and more scarce and thus expensive, it is important to explore alternative options to these chemicals, especially for academic applications. One such alternative is the use of stable isotope-labeled complex media from Silantes: All Silantes cell growth media are prepared from uniformly labeled bacterial Cupriavidus necator cell hydrolysate. This isotopically labeled biomass is a perfect nutrient medium for bacteria and yeasts, eliminating the need for expensive ¹³C glucose or D₂O.

More Information

Find out more about the D₂O and ¹³C-glucose price dynamics in our article “Why has the price of stable isotopes skyrocketed?”