SILAM Mouse

Stable isotopic labeling is the most reliable and accurate method for quantitative proteomics and metabolomics. The SILAM (Stable Isotope Labeling of Mammals) method is a well-established in-vivo labeling approach for relative tissue protein and metabolite quantification by mass spectrometry. This approach has been successfully applied to the analysis of mouse organs.

With SILAM, the concept of SILAC (Stable Isotope Labeling of Amino acids in Cell Culture) can be applied to living organisms.

The SILAM approach is commonly used for

• Relative protein or metabolite quantification by mass spectrometry,
• Global protein turnover analysis by mass spectrometry,
• Molecular structure confirmation and
• Biomarker discovery and verification.

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The central element of the SILAM method is the “spike-in” approach: Differences in protein patterns of unlabeled case tissue with respect to unlabeled control tissue can be quantified by “spiking-in” a stable-isotope-labeled reference tissue.

This stable-isotope labeled reference tissue can either be cultivated using the appropriate feed (see SILAM diets), or it can be obtained directly from Silantes (see SILAM Mouse Tissues).