NMR of Proteins

Stable isotope labeling of proteins is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy. Atoms in the protein are replaced with stable isotopes of carbon (¹³C), nitrogen (¹⁵N), and hydrogen (²H). This allows one to differentiate protein signals from other sample components. The resulting NMR spectra can provide detailed information about the protein’s structure, dynamics, and interactions, making stable isotope labeling of proteins a powerful tool in structural biology and drug discovery.

Labeling by different techniques

Stable isotope labeling of proteins can be achieved using a variety of techniques. The most commonly used methods are based on expressing the protein in a host organism, such as bacteria, yeast, insect cells, or mammalian cells using stable isotope labeled growth media. Alternatively, for small peptides, chemical solid-phase synthesis using stable isotope labeled amino acids can be used.

Our offer

At Silantes, we specialize in creating our own stable isotope-labeled growth media for various organisms. Additionally, we offer a wide range of isotope-labeled amino acids and model proteins with NMR quality. These products are valuable for research and experiments in the field of molecular biology and biochemistry.