Cell Growth Media for Escherichia coli and Yeast Culture

Structural studies on proteins using nuclear magnetic resonance (NMR) require labeling with stable isotopes. Silantes provides a range of stable isotope-labeled, rich cell growth media specifically formulated for the cultivation and optimal growth of Escherichia coli strains (E. coli) and yeast cells, and for the expression of stable isotope-labeled proteins. The media are available in all 2H, 13C, and 15N combinations.

Isotope-Labeled Culture Growth Media – Tailored to Your Needs

Depending on your application, we offer different media variations for the expression of stable isotope-labeled 2H, 13C, and 15N proteins in E. coli or yeast culture.

Concentrate rich growth media for E. coli and yeast

Media Concentrate Stocks give maximum flexibility allowing you to dilute to the desired concentration.

Silantes Concentrate Medium for Escherichia coli and yeast culture is a 10-fold concentrated medium obtained from the hydrolysates of stable isotope-labeled bacteria fermentation Cupriavidus nekator. This hydrolysate is a perfect nutrient source for bacteria and yeasts.

OD10 10-fold Concentrate Media

  • Unlabeled OD10 10-fold Concentrate Media Solution
  • 2H – OD10 10-fold Concentrate Media Solution
  • 13C – OD10 10-fold Concentrate Media Solution
  • 15N – OD10 10-fold Concentrate Media Solution
  • 2H, 15N – OD10 10-fold Concentrate Media Solution
  • 13C, 15N – OD10 10-fold Concentrate Media Solution
  • 2H, 13C, 15N – OD10 10-fold Concentrate Media Solution

High Performance OD2 Rich Growth Solution Media

OD2 Media give maximum performance. Optimised to an optical density of OD600 > 2 within 24 hours in a sterile ready-to-use solution.

The Silantes OpticalDensity2 Solution Medium represents the high-performance product amongst the Silantes stable isotope-labeled cell growth media. It can be used for the homologous or heterologous expression of isotopically labeled proteins in bacteria or yeast. Learn more about the Silantes OD2 media.

Culture growth media for E. coli

  • Unlabeled High Performance OD2 Media for E.coli
  • 2H – High Performance OD2 Media for E.coli
  • 13C – High Performance OD2 Media for E.coli
  • 15N – High Performance OD2 Media for E.coli
  • 2H, 15N – High Performance OD2 Media for E.coli
  • 13C, 15N – High Performance OD2 Media for E.coli
  • 2H, 13C, 15N – High Performance OD2 Media for E.coli

Culture growth media for yeast

  • Unlabeled High Performance OD2 Media for yeast
  • 2H – High Performance OD2 Media for yeast
  • 13C – High Performance OD2 Media for yeast
  • 15N – High Performance OD2 Media for yeast
  • 2H, 15N – High Performance OD2 Media for yeast
  • 13C, 15N – High Performance OD2 Media for yeast
  • 2H, 13C, 15N – High Performance OD2 Media for yeast

SILEX and ECO Powder Media for E. coli and yeast

SILEX and ECO Powder Media give maximum cost efficiency allowing you to dilute to the desired concentration and to boost M9 experiments performance.

Silantes SILEX and ECO media are powder cultivation medium made from Silantes OD2 medium. The powder media have been developed in order to combine the performance of the OD2 medium with a good shelf life.

SILEX Powder Media for E. coli

Bacterial growth media for the expression of stable isotope labeled protein. Isotopic enrichment > 98 atom%.

  • unlabeled SILEX Media powder for E.coli
  • 2H – SILEX Media powder for E.coli
  • 13C – SILEX Media powder for E.coli
  • 15N – SILEX Media powder for E.coli
  • 2H, 15N – SILEX Media powder for E.coli
  • 13, 15N – SILEX Media powder for E.coli
  • 2H, 13, 15N – SILEX Media powder for E.coli

ECO Powder Media for E. coli

Bacterial growth media for the expression of stable isotope labeled protein. Isotopic enrichment > 98 atom%.

  • 13C – ECO Media powder for E.coli
  • 13C 15N – ECO Media powder for E.coli

SILEX Powder Media for yeast

Yeast culture media for the expression of stable isotope labeled protein. Isotopic enrichment > 98 atom%.

  • unlabeled SILEX Media powder for yeast
  • 2H – SILEX Media powder for yeast
  • 13C – SILEX Media powder for yeast
  • 15N – SILEX Media powder for yeast
  • 2H, 15N – SILEX Media powder for yeast
  • 13C, 15N – SILEX Media powder for yeast
  • 2H, 13C, 15N – SILEX Media powder for yeast

ECO Powder Media for yeast

Yeast culture media for the expression of stable isotope labeled protein. Isotopic enrichment > 98 atom%.

  • 13C- ECO Media powder for yeast
  • 13C 15N – ECO Media powder for yeast

Rich Media compared to Minimal Media

A rich medium is a cell culture medium that contains, among other things, amino acids as well as glucose to promote protein expression.

A minimal medium, on the other hand, is a cell culture medium in which the only carbon source is glucose, glycerol or pyruvate, while the only nitrogen source is either ammonium chloride or ammonium sulphate.

In order to evaluate the cost-effectiveness of Silantes Rich media for your project, the respective costs of a corresponding minimal medium in relation to the protein yield can be used for comparison. We have validated that Silantes Rich Medium can increase protein expression by over 200% compared to Minimal Medium for numerous proteins.

Please contact us for a free sample to validate Silantes Rich Media for your application .

Boost the performance of Minimal Media with Silantes Rich Media

Stable isotope-labeled proteins are often expressed in minimal media (or “M9” media). As minimal media contains only essential nutrients required for growth, this can result in slower growth rates and lower biomass compared to rich media, ultimately leading to lower protein yields. This problem can be solved by supplementing the M9 medium with Silantes Rich Media.

Figure 1 demonstrates that 1 % w/v SILEX Powder Medium (or corresponding 1.7 % v/v OD10 Concentrate Medium) added as a supplement to M9 medium increases the initial cell growth rate and the final optical density.

Enhance your stable isotope labeled cell growth and protein yield in minimal media up to 300% in under 10 hours by adding 1% SILEX Powder Medium from Silantes.
Growth of E.coli BL 21 (pRSET-UB) in M9 medium with and without Silantes SILEX medium supplement.

High Standards in Quality Control

All our growth media have an isotope enrichment of >98 atom %. This specification is validated through mass spectrometry.

In addition, we ensure the biological integrity of our media by performing bacterial and yeast growth tests on each and every batch of media. Each delivery includes a quality certificate that includes all quality control results.

No Addition of D2O or 13C-Glucose Needed

As the raw materials D2O and 13C-Glucose become more and more scarce and thus expensive, it is important to explore alternative options to these chemicals, especially for academic applications. One such alternative is the use of stable isotope-labeled complex media from Silantes: All Silantes cell growth media are prepared from uniformly labeled bacterial Cupriavidus necator cell hydrolysate. This isotopically labeled biomass is a perfect nutrient medium for bacteria and yeasts, eliminating the need for expensive 13C glucose or D2O.

More Information

Find out more about the D2O and 13C-glucose price dynamics in our article โ€œWhy has the price of stable isotopes skyrocketed?”

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