SILAM Fly

Stable isotopic labeling is the most reliable and accurate method for quantitative proteomics and metabolomics. The SILAM (Stable Isotope Labeling of Mammals) method is a well-established in-vivo labeling approach for relative tissue protein and metabolite quantification by mass spectrometry.

Analogous to the “SILAM Mouse” concept, the approach has been successfully applied to the analysis of fly organs.

SILAM Fly Powder Diets

A prerequisite for the application of the in-vivo SILAM approach is the availability of an appropriate SILAM diet. Silantes has developed various unlabeled and stable isotope-labeled diets for flies allowing different labeling strategies:

• • Amino acid specific labeled SILAM powder diets for flies (suitable for proteomics studies)
  • ¹⁵N labeled SILAM powder diets for flies (suitable for proteomics and metabolomics studies)

  Both powder diets are based on Silantes-produced stable isotope-labeled biomass of the organism Saccharomyces cerevisiae.

  Ready-to-use SILAM Fly Gel Diets

  For easier handling, our powder diets are also available as ready-to-use agar gels. The gels are gamma-sterilized and contain glucose, nipagin and propionic acid in addition to the Silantes-produced isotope-labeled Saccharomyces cerevisiae biomass.

  Read more about the different in-vivo feeding strategies for SILAM
  Read more about the SILAM spike-in workflow

• References

  Use cases of the SILAM fly diet from Silantes in scientific publications:

  • Silantes Lys6-SILAM fly diet: Yang, L., Cao, Y., Zhao, J., Fang, Y., Liu, N., & Zhang, Y. (2019). Multidimensional proteomics identifies declines in protein homeostasis and mitochondria as early signals for normal aging and age-associated disease in Drosophila*[s]. Molecular & Cellular Proteomics, 18(10), 2078–2088. https://doi.org/10.1074/mcp.ra119.001621
  • Silantes Lys6-SILAM fly diet: Zaijun Ma Hui Wang Yuping Cai Han Wang Kongyan Niu Xiaofen Wu Huanhuan Ma Yun Yang Wenhua Tong Feng Liu Zhandong Liu Yaoyang Zhang Rui Liu Zheng-Jiang Zhu Nan Liu (2018) Epigenetic drift of H3K27me3 in aging links glycolysis to healthy longevity in Drosophila eLife 7:e35368. https://doi.org/10.7554/eLife.35368