Deuterium Oxide

Our heavy water undergoes extensive quality testing before release.

• Isotopic purity is validated using a Fourier Transform Infrared Spectrometer.
• For the use of heavy water in cell culture, each batch is tested for biological competence by performing growth tests.
• All analytical data are available upon request and are provided with each order.

Silantes D₂O quality specifications

The following table shows the minimum specifications for our D₂O sales release.

Test	Specification
Appearance	Clear, odorless liquid
FT-IR for Isotopic enrichment	> 99.9 or 99.8 or 98.0 atom % D
Conductivity (at 25 °C)	< 2 µS/cm
Organic components	< 10 mg KMnO / kg D2O
Turbidity	< 3 NTU
Chlorides	< 0.2 mg/kg
Sodium	< 0.2 mg/kg
Nitrates	< 0.1 mg/kg
Boron	< 0.1 mg/kg
Gadolinium	< 0.1 mg/kg
Iron	0.5 mg/kg
Tritium	< 3.7 x 104 Bq/l
Growth test for biological competence	OD 2 within 24 h

References

Use cases of the D2O from Silantes in scientific publications:

• Kselíková, V., Husarčíková, K., Mojzeš, P., Zachleder, V., & Bišová, K. (2022). Cultivation of the microalgae Chlamydomonas reinhardtii and Desmodesmus quadricauda in highly deuterated media: Balancing the light intensity. Frontiers in Bioengineering and Biotechnology, 10. https://doi.org/10.3389/fbioe.2022.960862
• Frank, S., Reglitz, K., Mall, V., Morgenstern, U., & Steinhaus, M. (2021). Molecular background of the undesired odor of polypropylene materials and insights into the sources of key odorants. Indoor Air, 31(4), 1038–1049. https://doi.org/10.1111/ina.12821
• Nedbal, J., Gao, L., & Suhling, K. (2020). Bottom-illuminated orbital shaker for microalgae cultivation. HardwareX, 8, e00143. https://doi.org/10.1016/j.ohx.2020.e00143
• Singh, A. K., Balchin, D., Imamoglu, R., Hayer-Hartl, M., & Hartl, F. U. (2020). Efficient catalysis of protein folding by GROEL/ES of the obligate chaperonin substrate METF. Journal of Molecular Biology, 432(7), 2304–2318. https://doi.org/10.1016/j.jmb.2020.02.031