111701402
111701402

High Performance OD2 Media solution for Yeast

From: 220 € plus VAT, plus delivery

Available in various isotopic labelings and/or quantities.

SKU: OD2-Y Categories: , , , ,

Description

– This product is in a ready-to-use sterile solution

– Isotopic enrichment for all labelings except for U-2H: > 98 atom %

– Isotopic enrichment for U-2H: > 97 atom %

The media are made from bacterial hydrolysate and contain primarily amino acids, some low molecular weight oligopeptides and almost no carbohydrates. The bacterial strain used is a chemolithoautotrophic organism which grows on inorganic substrates that are isotopically labeled.

Note: Silantes media are natural products. A slightly different color (e.g. amongst different batches) is absolutely normal for natural products.

Silantes OD2 media are rich growth media for a high-performance expression of stable isotope labeled proteins. The media are formulated for a > OD2 cell density (optical density measured at 600nm).

References

References

RELEVANT DOCUMENTS:

• Overview Stable Isotope Labeled Cell Growth Media: https://silantes-products.com/product_catalogue/Silantes_E.coli_Yeast_Overview_Cell_Growth_Media.pdf
• Silantes OpticalDensity2 Solution Medium: https://silantes-products.com/product_catalogue/Silantes_E.coli_Yeast_High_Performance_OD2_Media.pdf
• Silantes SILEX and ECO Powder Media: https://silantes-products.com/product_catalogue/Silantes_E.coli_Yeast_SILEX_ECO_Powder_Media.pdf
• Silantes Concentrate Medium: https://silantes-products.com/product_catalogue/Silantes_E.coli_Yeast_Concentrate_OD10_Media.pdf
• Boost Protein Expression in M9 with Silantes Media: https://silantes-products.com/product_catalogue/Silantes_Boost_Protein_Expression_in_M9_with_Silantes_Media.pdf

RELEVANT MANUALS:
• Expression of Stable Isotope Labeled Protein with Silantes Cell Growth Media for E.coli: https://silantes-products.com/product_catalogue/Silantes_E.coli_Yeast_Manual_for_protein_expression_media_for_e.coli.pdf
• Expression of Stable Isotope Labeled Protein with Silantes Cell Growth Media for yeast: https://silantes-products.com/product_catalogue/Silantes_E.coli_Yeast_Manual_for_protein_expression_media_for_yeast.pdf

USE CASES OF THE SILANTES OD2 MEDIUM IN SCIENTIFIC PUBLICATIONS:

• Mallagaray, A., Creutznacher, R., Dülfer, J., Mayer, P. H. O., Grimm, L. L., Orduña, J. M., … Peters, T. (2019). A post-translational modification of human Norovirus capsid protein attenuates glycan binding. Nature Communications, 10(1). https://doi.org/10.1038/s41467-019-09251-5
• Niesteruk, A., Jonker, H. R. A., Richter, C., Linhard, V., Sreeramulu, S., & Schwalbe, H. (2018). The domain architecture of PtkA, the first tyrosine kinase from Mycobacterium tuberculosis, differs from the conventional kinase architecture. Journal of Biological Chemistry, 293(30), 11823–11836. https://doi.org/10.1074/jbc.ra117.000120
• Pimienta, G. (2004). Structural characterization of a protein/RNA complex : human TAP/NXF1 protein/retroviral CTE RNA. Heidelberg University Library. https://doi.org/10.11588/HEIDOK.00005077 • Borland, K., Diesend, J., Ito-Kureha, T., Heissmeyer, V., Hammann, C., Buck, A. H., … Kellner, S. (2019). Production and Application of Stable Isotope-Labeled Internal Standards for RNA Modification Analysis. Genes, 10(1), 26. https://doi.org/10.3390/genes10010026

USE CASES OF THE SILANTES SILEX POWDER MEDIUM IN SCIENTIFIC PUBLICATIONS:

• 2H 13C 15N SILEX Medium for E.coli as substitute to M9: Movellan, K. T., Najbauer, E. E., Pratihar, S., Salvi, M., Giller, K., Becker, S., & Andreas, L. B. (2019). Alpha protons as NMR probes in deuterated proteins. Journal of Biomolecular NMR, 73(1–2), 81–91. https://doi.org/10.1007/s10858-019-00230-y
• 2H 13C 15N OD2 Medium for E.coli as substitute to M9: Botuyan, M. V. E., Nominé, Y., Yu, X., Juranic, N., Macura, S., Chen, J., & Mer, G. (2004). Structural basis of BACH1 phosphopeptide recognition by BRCA1 tandem BRCT domains. Structure, 12(7), 1137–1146. https://doi.org/10.1016/j.str.2004.06.002
• 2H 13C 15N OD2 Medium for E.coli as substitute to M9: Lingel, A., Simon, B., Izaurralde, E., & Sattler, M. (2005). The structure of the flock house virus B2 protein, a viral suppressor of RNA interference, shows a novel mode of double‐stranded RNA recognition. EMBO Reports, 6(12), 1149–1155. https://doi.org/10.1038/sj.embor.7400583

RELEVANT BLOG ARTICLES:

• Why has the stable isotope price skyrocketed?: https://www.silantes.com/why-has-the-price-of-stable-isotopes-skyrocketted/
• Stable isotope labeled rich growth media: https://www.silantes.com/stable-isotope-labelled-rich-growth-media/
• Applying Silantes Cell Growth Media to study Human RNA Modification Dynamics: https://www.silantes.com/silantes-cell-growth-media-to-study-human-rna-modification-dynamics/

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