SILAC RPMI Kits

From: 290  plus VAT, plus delivery

Available in various isotopic labelings.
Quantity: Kit

Description

– Isotopic enrichment for the stable isotope labeled amino acids except for 4, 4, 5, 5, D4 L-lysine: > 98 atom %

– Isotopic enrichment for for 4, 4, 5, 5, D4 L-lysine: > 96 atom %

Dry ice shipment required.

References

Relevant manuals:

โ€ข Silantes Powder RPMI and DMEM for SILAC Preparation of sterile liquid Cell Culture Media: https://silantes.com/documents/manuals/Silantes_SILAC_RPMI_DMEM_Powder_Application_Note.pdf

SILAC amino acids from Silantes in scientific publications:

โ€ข Hao, B., Li, X., Jia, X., Wang, Y., Zhai, L., Li, D., Liu, J., Zhang, D., Chen, Y., Xu, Y., Lee, S., Xu, G., Chen, X., Dang, Y., Liu, B., & Tan, M. (2020). The novel cereblon modulator CC-885 inhibits mitophagy via selective degradation of BNIP3L. Acta Pharmacologica Sinica, 41(9), 1246โ€“1254. https://doi.org/10.1038/s41401-020-0367-9

โ€ข LรถรŸner, C., Warnken, U., Pscherer, A., & Schnรถlzer, M. (2011). Preventing arginine-to-proline conversion in a cell-line-independent manner during cell cultivation under stable isotope labeling by amino acids in cell culture (SILAC) conditions. Analytical Biochemistry, 412(1), 123โ€“125. https://doi.org/10.1016/j.ab.2011.01.011

โ€ข Sigismondo, G., Arseni, L., Palacio-Escat, N., Hofmann, T. G., Seiffert, M., & Krijgsveld, J. (2023c). Multi-layered chromatin proteomics identifies cell vulnerabilities in DNA repair. Nucleic Acids Research, 51(2), 687โ€“711. https://doi.org/10.1093/nar/gkac1264

โ€ข Pateetin, P., Hutvagner, G., Bajan, S., Padula, M. P., McGowan, E. M., & Boonyaratanakornkit, V. (2021). Triple SILAC identified progestin-independent and dependent PRA and PRB interacting partners in breast cancer. Scientific Data, 8(1). https://doi.org/10.1038/s41597-021-00884-0

โ€ข Lopez-Serra, P., Marcilla, M., Villanueva, A., Ramos-Fernandez, A., Palau, A., Leal, L., Wahi, J. E., Setien-Baranda, F., Szczesna, K., Moutinho, C., Martinez-Cardus, A., Heyn, H., Sandoval, J., Puertas, S., Vidal, A., Sanjuan, X., Martinez-Balibrea, E., Viรฑals, F., Perales, J. C., . . . Esteller, M. (2014). A DERL3-associated defect in the degradation of SLC2A1 mediates the Warburg effect. Nature Communications, 5(1). https://doi.org/10.1038/ncomms4608

โ€ข Ong, S., Kratchmarova, I., & Mann, M. (2002). Properties of 13C-Substituted arginine in stable isotope labeling by amino acids in cell culture (SILAC). Journal of Proteome Research, 2(2), 173โ€“181. https://doi.org/10.1021/pr0255708

โ€ข LรถรŸner, C., Warnken, U., Pscherer, A., & Schnรถlzer, M. (2011b). Preventing arginine-to-proline conversion in a cell-line-independent manner during cell cultivation under stable isotope labeling by amino acids in cell culture (SILAC) conditions. Analytical Biochemistry, 412(1), 123โ€“125. https://doi.org/10.1016/j.ab.2011.01.011

โ€ขMalet, J. K., Impens, F., Carvalho, F., Hamon, M. A., Cossart, P., & Ribet, D. (2018b). Rapid remodeling of the host epithelial cell proteome by the listeriolysin O (LLO) pore-forming toxin. Molecular & Cellular Proteomics, 17(8), 1627โ€“1636. https://doi.org/10.1074/mcp.ra118.000767

โ€ข Rogers, L. C., Kremer, J. C., Brashears, C. B., Lin, Z., Hu, Z., Bastos, A. C., Baker, A., Fettig, N., Zhou, D., Shoghi, K. I., Dehner, C. A., Chrisinger, J. S., Bomalaski, J. S., Garcia, B. A., Oyama, T., White, E. P., & Van Tine, B. A. (2023). Discovery and targeting of a noncanonical mechanism of sarcoma resistance to ADI-PEG20 mediated by the microenvironment. Clinical Cancer Research, 29(16), 3189โ€“3202. https://doi.org/10.1158/1078-0432.ccr-22-2642

โ€ข Geiger, T., Wisniewski, J. R., Cox, J., Zanivan, S., Kruger, M., Ishihama, Y., & Mann, M. (2011). Use of stable isotope labeling by amino acids in cell culture as a spike-in standard in quantitative proteomics. Nature Protocols, 6(2), 147โ€“157. https://doi.org/10.1038/nprot.2010.192

โ€ข Hao, B., Sun, M., Zhang, M., Zhao, X., Zhao, L., Li, B., Zhai, L., Liu, P., Hu, H., Xu, J., & Tan, M. (2020). Global characterization of proteome and lysine methylome features in EZH2 wild-type and mutant lymphoma cell lines. Journal of Proteomics, 213, 103614. https://doi.org/10.1016/j.jprot.2019.103614

Relevant blog articles:

โ€ข Quantitative Proteomics Explained: Techniques, Applications, and Challenges: https://www.silantes.com/guide-to-quantitative-proteomics-techniques-applications-and-challenges/

โ€ข Quantitative Proteomics: Comparing the Big Three โ€“ iTRAQ, TMT, and SILAC: https://www.silantes.com/itraq-tmt-silac/

โ€ข Quantitative Proteomics: Label-Free versus Label-Based Methods: https://www.silantes.com/label-vs-label-free-quantitative-proteomics/

โ€ข Understanding the Role of Mass Spectrometry in Metabolomics: https://www.silantes.com/mass-spectrometry-metabolomics/

โ€ข Understanding Proteomics: A Comprehensive Guide to the Various Types: https://www.silantes.com/types-of-proteomics/

Relevant webinars:

โ€ข Proteomics using SILAC presented by Prof. Dr. Marcus Krรผger: https://www.youtube.com/watch?v=TY6iV8_43iA

โ€ข Multiplexing with isotopic labels for DDA and DIA in MaxQuant presented by Dr. Jรผrgen Cox: https://www.youtube.com/watch?v=-Yddv7ehYsk

โ€ข Stable Isotope Labeling of Mammals presented by Dr. Christoph Turck: https://www.youtube.com/watch?v=BQWvLdILjCA