120703804
120703804

Thymidine 5′-triphosphate

From: 110 € plus VAT, plus delivery

dTTP Li2-salt

Available in various isotopic labelings and/or quantities.

SKU: DTTP Categories: , , , , , , , , ,

Description

– This product is in solution

– Concentration: c=0.1 mol/L

– Buffer information: 5 mM TrisHCl pH 7.5 / H2O

– Buffer information for deuterated Products: 5mM TrisHCl pH 7.5 / D2O

– Isotopic enrichment for all labelings except for U-2H: > 98 atom %

– Isotopic enrichment for U-2H: > 96 atom %

– Chemical purity > 95 %

Dry ice shipment required.

References

Relevant documents:

• Stable Isotope-Labeled RNA and DNA Building Blocks & Oligonucleotide Synthesis Service at Silantes: https://silantes-products.com/product_catalogue/Silantes_Stable_Isotope_RNA_and_DNA_building_blocks_and_synthesis_service.pdf

Silantes NTPs in scientific publications

• Mieczkowski, M., Steinmetzger, C., Bessi, I., Lenz, A., Schmiedel, A., Holzapfel, M., Lambert, C., Pena, V., & Höbartner, C. (2021). Large Stokes shift fluorescence activation in an RNA aptamer by intermolecular proton transfer to guanine. Nature Communications, 12(1). https://doi.org/10.1038/s41467-021-23932-0 [Titel anhand dieser DOI in Citavi-Projekt übernehmen]

• Musheev, M. U., Schomacher, L., Basu, A., Han, D., Krebs, L., Scholz, C., & Niehrs, C. (2022). Mammalian N1-adenosine PARylation is a reversible DNA modification. Nature Communications, 13(1). https://doi.org/10.1038/s41467-022-33731-w [Titel anhand dieser DOI in Citavi-Projekt übernehmen]

• Xu, Y., McSally, J., Andricioaei, I., & Al-Hashimi, H. M. (2018). Modulation of Hoogsteen dynamics on DNA recognition. Nature Communications, 9(1). https://doi.org/10.1038/s41467-018-03516-1 [Titel anhand dieser DOI in Citavi-Projekt übernehmen]

• Li, M., Wang, Y., Wei, X., Cai, W., Wu, J., Zhu, M., Wang, Y., Liu, Y., Xiong, J., Qu, Q., Chen, Y., Tian, X., Yao, L., Xie, R., Li, X., Chen, S., Huang, X., Zhang, C., Xie, C., . . . Lin, S. (2024). AMPK targets PDZD8 to trigger carbon source shift from glucose to glutamine. Cell Research. https://doi.org/10.1038/s41422-024-00985-6 [Titel anhand dieser DOI in Citavi-Projekt übernehmen]

• Cromsigt, J., Schleucher, J., Gustafsson, T., Kihlberg, J., & Wijmenga, S. (2002). Preparation of partially 2H/13C-labelled RNA for NMR studies. Stereo-specific deuteration of the H5’’ in nucleotides. Nucleic Acids Research, 30(7), 1639–1645. https://doi.org/10.1093/nar/30.7.1639 [Titel anhand dieser DOI in Citavi-Projekt übernehmen]

• Rangadurai, A., Szymanski, E. S., Kimsey, I., Shi, H., & Al-Hashimi, H. M. (2020). Probing conformational transitions towards mutagenic Watson–Crick-like G·T mismatches using off-resonance sugar carbon R1ρ relaxation dispersion. Journal of Biomolecular NMR, 74(8–9), 457–471. https://doi.org/10.1007/s10858-020-00337-7 [Titel anhand dieser DOI in Citavi-Projekt übernehmen]

• Noeske, J., Richter, C., Grundl, M. A., Nasiri, H. R., Schwalbe, H., & Wöhnert, J. (2005). An intermolecular base triple as the basis of ligand specificity and affinity in the guanine- and adenine-sensing riboswitch RNAs. Proceedings of the National Academy of Sciences, 102(5), 1372–1377. https://doi.org/10.1073/pnas.0406347102 [Titel anhand dieser DOI in Citavi-Projekt übernehmen]

• Ohira, T., Minowa, K., Sugiyama, K., Yamashita, S., Sakaguchi, Y., Miyauchi, K., Noguchi, R., Kaneko, A., Orita, I., Fukui, T., Tomita, K., & Suzuki, T. (2022). Reversible RNA phosphorylation stabilizes tRNA for cellular thermotolerance. Nature, 605(7909), 372–379. https://doi.org/10.1038/s41586-022-04677-2 [Titel anhand dieser DOI in Citavi-Projekt übernehmen]

• Vögele, J., Duchardt-Ferner, E., Bains, J. K., Knezic, B., Wacker, A., Sich, C., Weigand, J. E., Šponer, J., Schwalbe, H., Krepl, M., & Wöhnert, J. (2024). Structure of an internal loop motif with three consecutive U•U mismatches from stem–loop 1 in the 3′-UTR of the SARS-CoV-2 genomic RNA. Nucleic Acids Research, 52(11), 6687–6706. https://doi.org/10.1093/nar/gkae349 [Titel anhand dieser DOI in Citavi-Projekt übernehmen]

• Broft, P., Rosenkranz, R. R., Schleiff, E., Hengesbach, M., & Schwalbe, H. (2022). Structural analysis of temperature-dependent alternative splicing of HsfA2 pre-mRNA from tomato plants. RNA Biology, 19(1), 266–278. https://doi.org/10.1080/15476286.2021.2024034 [Titel anhand dieser DOI in Citavi-Projekt übernehmen]

Silantes phosphoramidites in scientific publications:

• Becette, O., Olenginski, L. T., & Dayie, T. K. (2019). Solid-Phase chemical synthesis of stable Isotope-Labeled RNA to aid structure and dynamics studies by NMR spectroscopy. Molecules, 24(19), 3476. https://doi.org/10.3390/molecules24193476 [Titel anhand dieser DOI in Citavi-Projekt übernehmen]

• Štih, V., Amenitsch, H., Plavec, J., & Podbevšek, P. (2023). Spatial arrangement of functional domains in OxyS stress response sRNA. RNA, 29(10), 1520–1534. https://doi.org/10.1261/rna.079618.123 [Titel anhand dieser DOI in Citavi-Projekt übernehmen]

Silantes oligonucleotide synthesis service in scientific publications:

• Belfetmi, A., Zargarian, L., Tisné, C., Sleiman, D., Morellet, N., Lescop, E., Maskri, O., René, B., Mély, Y., Fosse, P., & Mauffret, O. (2016). Insights into the mechanisms of RNA secondary structure destabilization by the HIV-1 nucleocapsid protein. RNA, 22(4), 506–517. https://doi.org/10.1261/rna.054445.115 [Titel anhand dieser DOI in Citavi-Projekt übernehmen]

• Borggräfe, J., Victor, J., Rosenbach, H., Viegas, A., Gertzen, C. G. W., Wuebben, C., … Etzkorn, M. (2021). Time-resolved structural analysis of an RNA-cleaving DNA catalyst. Nature, 601(7891), 144–149. https://doi.org/10.1038/s41586-021-04225-4 [Titel anhand dieser DOI in Citavi-Projekt übernehmen]

• Chernatynskaya, A. V., Deleeuw, L., Trent, J. O., Brown, T., & Lane, A. N. (2009). Structural analysis of the DNA target site and its interaction with Mbp1. Organic & Biomolecular Chemistry, 7(23), 4981. https://doi.org/10.1039/b912309a [Titel anhand dieser DOI in Citavi-Projekt übernehmen]

• Van Melckebeke, H., Devany, M., Di Primo, C., Beaurain, F., Toulmé, J., Bryce, D. L., & Boisbouvier, J. (2008). Liquid-crystal NMR structure of HIV TAR RNA bound to its SELEX RNA aptamer reveals the origins of the high stability of the complex. Proceedings of the National Academy of Sciences, 105(27), 9210–9215. https://doi.org/10.1073/pnas.0712121105 [Titel anhand dieser DOI in Citavi-Projekt übernehmen]

Silantes 14-mer RNA Silantes 14-mer RNA Standard in scientific publications:Standard in scientific publications:

• Duchardt, E., & Schwalbe, H. (2005). Residue Specific Ribose and Nucleobase Dynamics of the cUUCGg RNA Tetraloop Motif by MNMR 13C Relaxation. Journal of Biomolecular NMR, 32(4), 295–308. https://doi.org/10.1007/s10858-005-0659-x [Titel anhand dieser DOI in Citavi-Projekt übernehmen]

• Hartlmüller, C., Günther, J. C., Wolter, A. C., Wöhnert, J., Sattler, M., & Madl, T. (2017). RNA structure refinement using NMR solvent accessibility data. Scientific Reports, 7(1). https://doi.org/10.1038/s41598-017-05821-z [Titel anhand dieser DOI in Citavi-Projekt übernehmen]

• Nozinovic, S., Fürtig, B., Jonker, H. R. A., Richter, C., & Schwalbe, H. (2009). High-resolution NMR structure of an RNA model system: the 14-mer cUUCGg tetraloop hairpin RNA. Nucleic Acids Research, 38(2), 683–694. https://doi.org/10.1093/nar/gkp956 [Titel anhand dieser DOI in Citavi-Projekt übernehmen]

• Richter, C., Kovacs, H., Buck, J., Wacker, A., Fürtig, B., Bermel, W., & Schwalbe, H. (2010). 13C-direct detected NMR experiments for the sequential J-based resonance assignment of RNA oligonucleotides. Journal of Biomolecular NMR, 47(4), 259–269. https://doi.org/10.1007/s10858-010-9429-5 [Titel anhand dieser DOI in Citavi-Projekt übernehmen]

• Ferner, J., Villa, A., Duchardt, E., Widjajakusuma, E., Wöhnert, J., Stock, G., & Schwalbe, H. (2008). NMR and MD studies of the temperature-dependent dynamics of RNA YNMG-tetraloops. Nucleic Acids Research, 36(6), 1928–1940. https://doi.org/10.1093/nar/gkm1183

Relevant blog articles:

• Applications of Stable Isotope-Labeled Molecules: Exploring the Power of Isotopic Tracers: https://www.silantes.com/applications-stable-isotope-labeled-molecules/

• Custom RNA & DNA Synthesis Services: Tailored Solutions for Your Nucleic Acid Needs: https://www.silantes.com/custom-dna-rna-synthesis/

Relevant webinars:

• Easy 13C, 15N-Labeling of DNA through isothermal amplification: Applications to G-quadruplex, aptamer, and DNAzyme: https://www.youtube.com/watch?v=HDOHcVhjZro

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