151703201
151703201

Ubiquitin in sealed 5 mm NMR-tube

From: 460 € plus VAT, plus delivery

Available in various isotopic labelings and/or quantities.

SKU: UBIQ-TUBE Categories: , , ,

Description

This product is in sterile solution in sealed NMR-tube.

 

Buffer information:

0.5 mM in 0.55 mL 7 % D2O / 93 % H2O, 30 mM D-labeled NaAcetat, pH 4.7, 50 mM NaCl

 

NMR-Tube information:

535-PP-8, outside diameter 5 mm, 600 MHz

 

– Isotopic enrichment for all labelings except for U-2H: > 98 atom %

– Isotopic enrichment for U-2H: > 97 atom %

– Chemical purity > 95 %

– Recombinant, expressed in E.coli

Sequence: MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

References

Relevant documents:

• Stable Isotope Labeled Ubiquitin: https://silantes-products.com/product_catalogue/Silantes_Ubiquitin.pdf

NMR-Spectra of the ubiquitin from Silantes:

• Superimposed NMR spectra of 2H13C15N-labelled ubiquitin (blue) and 13C15N-labelled ubiquitin (red): https://silantes.com/documents/2D_NMR_Spectra_Ubiquitin/2d_15N-TROSY_cdn(blau)_cxn(rot).pdf

• Superimposed NMR spectra of 2H13C15N-labelled ubiquitin (blue) and 2H15N-labelled ubiquitin (red): https://silantes.com/documents/2D_NMR_Spectra_Ubiquitin/2d_15N-TROSY_cdn(blau)_xdn(rot).pdf

• Superimposed NMR spectra of 2H13C15N-labelled ubiquitin (blue) and 15N-labelled ubiquitin (red): https://silantes.com/documents/2D_NMR_Spectra_Ubiquitin/2d_15N-TROSY_cdn(blau)_xxn(rot).pdf

• Superimposed NMR spectra of 13C15N-labelled ubiquitin (blue) and 2H15N-labelled ubiquitin (red): https://silantes.com/documents/2D_NMR_Spectra_Ubiquitin/2d_15N-TROSY_cxn(blau)_xdn(rot).pdf

• Superimposed NMR spectra of 13C15N-labelled ubiquitin (blue) and 15N-labelled ubiquitin (red): https://silantes.com/documents/2D_NMR_Spectra_Ubiquitin/2d_15N-TROSY_cxn(blau)_xxn(rot).pdf

• Superimposed NMR spectra of 2H15N-labelled ubiquitin (blue) and 15N-labelled ubiquitin (red): https://silantes.com/documents/2D_NMR_Spectra_Ubiquitin/2d_15N-TROSY_xdn(blau)_xxn(rot).pdf

•Superimposed NMR spectra of 15N-labelled ubiquitin (blue), 2H15N-labelled ubiquitin (red), 13C15N-labelled ubiquitin (magenta) and 2H13C15N-labelled ubiquitin (yellow): https://silantes.com/documents/2D_NMR_Spectra_Ubiquitin/2d_15N-TROSY_xxn(blau)_xdn(rot)_cxn(magenta)_cdn(gelb).pdf

Silantes ubiquitin in scientific publications:

• Association between CSF alpha-synuclein seeding activity and genetic status in Parkinson’s disease and dementia with Lewy bodies. (n.d.). http://springermedizin.de . https://www.springermedizin.de/association-between-csf-alpha-synuclein-seeding-activity-and-gen/25667868

• Yuan, X., Simpson, P., Mckeown, C., Kondo, H., Uchiyama, K., Wallis, R., Dreveny, I., Keetch, C., Zhang, X., Robinson, C., Freemont, P., & Matthews, S. (2004). Structure, dynamics and interactions of p47, a major adaptor of the AAA ATPase, p97. The EMBO Journal, 23(7), 1463–1473. https://doi.org/10.1038/sj.emboj.7600152 [Titel anhand dieser DOI in Citavi-Projekt übernehmen]

•Haller, J. D., Bodor, A., & Luy, B. (2022). Pure shift amide detection in conventional and TROSY-type experiments of 13C,15N-labeled proteins. Journal of Biomolecular NMR, 76(5–6), 213–221. https://doi.org/10.1007/s10858-022-00406-z [Titel anhand dieser DOI in Citavi-Projekt übernehmen]

• Toft, A., Sjödin, S., Simonsen, A. H., Ejlerskov, P., Roos, P., Musaeus, C. S., Henriksen, E. E., Nielsen, T. T., Brinkmalm, A., Blennow, K., Zetterberg, H., & Nielsen, J. E. (2023). Endo‐lysosomal protein concentrations in CSF from patients with frontotemporal dementia caused by CHMP2B mutation. Alzheimer S & Dementia Diagnosis Assessment & Disease Monitoring, 15(1). https://doi.org/10.1002/dad2.12402 [Titel anhand dieser DOI in Citavi-Projekt übernehmen]

• Sjödin, S., Hansson, O., Öhrfelt, A., Brinkmalm, G., Zetterberg, H., Brinkmalm, A., & Blennow, K. (2017). Mass spectrometric analysis of cerebrospinal fluid ubiquitin in Alzheimer’s disease and parkinsonian disorders. PROTEOMICS – CLINICAL APPLICATIONS, 11(11–12). https://doi.org/10.1002/prca.201700100 [Titel anhand dieser DOI in Citavi-Projekt übernehmen]

• Lowe, A. J., Sjödin, S., Rodrigues, F. B., Byrne, L. M., Blennow, K., Tortelli, R., Zetterberg, H., & Wild, E. J. (2020). Cerebrospinal fluid endo-lysosomal proteins as potential biomarkers for Huntington’s disease. PLoS ONE, 15(8), e0233820. https://doi.org/10.1371/journal.pone.0233820 [Titel anhand dieser DOI in Citavi-Projekt übernehmen]

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