SILAM Mouse

With SILAM, the concept concept of SILAC (Stable Isotope Labeling of Amino acids in Cell Culture) can be applied to living organisms.

The SILAM approach is commonly used for

• Relative protein or metabolite quantification by mass spectrometry,
• Global protein turnover analysis by mass spectrometry,
• Molecular structure confirmation and
• Biomarker discovery and verification.

Selected products in this field (complete product range at bottom of page):

The central element of the SILAM method is the “spike-in” approach: Differences in protein patterns of unlabeled case tissue with respect to unlabeled control tissue can be quantified by “spiking-in” a stable-isotope-labeled reference tissue.

This stable-isotope labeled reference tissue can either be cultivated using the appropriate feed (see SILAM diets), or it can be obtained directly from Silantes (see SILAM Mouse Tissues).